Crystallization and peptide maps of neuraminidase “heads” from H2N2 and H3N2 influenza virus strains
Identifieur interne : 002A00 ( Main/Exploration ); précédent : 002999; suivant : 002A01Crystallization and peptide maps of neuraminidase “heads” from H2N2 and H3N2 influenza virus strains
Auteurs : W. G. Laver [Australie]Source :
- Virology [ 0042-6822 ] ; 1978.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Peptides, Protéines virales, Sialidase.
- enzymologie : Virus de la grippe A.
- isolement et purification : Sialidase.
- Cristallisation, Recombinaison génétique, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Spécificité d'espèce.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Neuraminidase, Peptides, Viral Proteins.
- enzymology : Influenza A virus.
- chemical , isolation & purification : Neuraminidase.
- Teeft :
- Academic press, Antigenic, Avian influenza virus, Crystalline material, Crystallization, Degraded, Degraded neuraminidase molecules, Digestion, Hemagglutinin, Hemagglutinin molecules, Hong kong, Hong kong influenza, Hong kong influenza virus, Hong kong neuraminidase, Hong kong strain, Hong strains, Influenza, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza virus, Influenza virus neuraminidase, Influenza virus strains, Influenza viruses, Intact neuraminidase molecules, Kilbourne, Laver, Molecular weight, Nacl, Neuraminidase, Neuraminidase activity, Neuraminidase crystals, Neuraminidase heads, Neuraminidase molecules, Peptide, Peptide mapping, Peptide mapping experiments, Peptide maps, Polypeptide, Pronase, Pronase digestion, Recombinant, Recombinant virus, Recombination, Genetic, Species Specificity, Sucrose, Surface antigens, Terminal members, Virology, Virus, Virus particles.
Abstract
Abstract: Neuraminidase “heads” of two H2N2 influenza virus strains (A/Nederlands/68 and A/Korea/68) and one H3N2 strain (A/Hong Kong/68) were isolated by pronase digestion of recombinant viruses possessing neuraminidase molecules from these strains and H (NWS) hemagglutinin. The neuraminidase heads were purified and crystallized by dialysis against distilled water. Large flat crystals, which differed in appearance between the three strains, were obtained. Tryptic peptide maps of the crystallized neuraminidase heads of the Nederlands/68 and Hong Kong/68 strains were almost identical, suggesting that a virus closely related to the Nederlands/68 (H2N2) strain may have been the source of the neuraminidase for the formation of the H3N2 Hong Kong subtype.
Url:
DOI: 10.1016/0042-6822(78)90009-0
Affiliations:
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G." last="Laver">W. G. Laver</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra</wicri:regionArea><wicri:noRegion>Canberra</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1978">1978</date><biblScope unit="volume">86</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="78">78</biblScope><biblScope unit="page" to="87">87</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus (enzymology)</term><term>Neuraminidase (analysis)</term><term>Neuraminidase (isolation & purification)</term><term>Peptides (analysis)</term><term>Recombination, Genetic</term><term>Species Specificity</term><term>Viral Proteins (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cristallisation</term><term>Peptides (analyse)</term><term>Protéines virales (analyse)</term><term>Recombinaison génétique</term><term>Sialidase (analyse)</term><term>Sialidase (isolement et purification)</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Spécificité d'espèce</term><term>Virus de la grippe A (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Neuraminidase</term><term>Peptides</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peptides</term><term>Protéines virales</term><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sialidase</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Antigenic</term><term>Avian influenza virus</term><term>Crystalline material</term><term>Crystallization</term><term>Degraded</term><term>Degraded neuraminidase molecules</term><term>Digestion</term><term>Hemagglutinin</term><term>Hemagglutinin molecules</term><term>Hong kong</term><term>Hong kong influenza</term><term>Hong kong influenza virus</term><term>Hong kong neuraminidase</term><term>Hong kong strain</term><term>Hong strains</term><term>Influenza</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Influenza virus strains</term><term>Influenza viruses</term><term>Intact neuraminidase molecules</term><term>Kilbourne</term><term>Laver</term><term>Molecular weight</term><term>Nacl</term><term>Neuraminidase</term><term>Neuraminidase activity</term><term>Neuraminidase crystals</term><term>Neuraminidase heads</term><term>Neuraminidase molecules</term><term>Peptide</term><term>Peptide mapping</term><term>Peptide mapping experiments</term><term>Peptide maps</term><term>Polypeptide</term><term>Pronase</term><term>Pronase digestion</term><term>Recombinant</term><term>Recombinant virus</term><term>Recombination, Genetic</term><term>Species Specificity</term><term>Sucrose</term><term>Surface antigens</term><term>Terminal members</term><term>Virology</term><term>Virus</term><term>Virus particles</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cristallisation</term><term>Recombinaison génétique</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Spécificité d'espèce</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Neuraminidase “heads” of two H2N2 influenza virus strains (A/Nederlands/68 and A/Korea/68) and one H3N2 strain (A/Hong Kong/68) were isolated by pronase digestion of recombinant viruses possessing neuraminidase molecules from these strains and H (NWS) hemagglutinin. The neuraminidase heads were purified and crystallized by dialysis against distilled water. Large flat crystals, which differed in appearance between the three strains, were obtained. Tryptic peptide maps of the crystallized neuraminidase heads of the Nederlands/68 and Hong Kong/68 strains were almost identical, suggesting that a virus closely related to the Nederlands/68 (H2N2) strain may have been the source of the neuraminidase for the formation of the H3N2 Hong Kong subtype.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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